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Cellulases production with pure and mixed cultures of Trichoderma reesei and Aspergillus fumigatus using rice husks as substrate
Producción de celulasas con cultivos puros y mixtos de Trichoderma reesei y Aspergillus fumigatus usando cascarilla de arroz como sustrato
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Cellulases production with pure and mixed cultures of Trichoderma reesei and Aspergillus fumigatus using rice husks as substrate. (2023). Revista EIA, 20(40), 4008 pp. 1-13. https://doi.org/10.24050/reia.v20i40.1671
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Cellulolytic enzymes are used in different industries; many of them are obtained from fungi
and bacteria that use agro-industrial waste with a high cellulose content as a substrate.
Some fungi of the genera Trichoderma and Aspergillus synthesize cellulases with different
mechanisms of action and mixed cultures of these fungi have been proposed as a strategy
to improve the hydrolysis of cellulosic materials associated with the production of these
enzymes. The objective of this study was to verify the production of cellulases from mixed
and pure cultures of native isolates of T. reesei and A. fumigatus with cellulolytic activity
using rice husks as a substrate. Both fungi produce enzymes that catalyze the release of
reducing sugars from carboxymethylcellulose. Solid state fermentation was carried out
following a factorial design and the effect of the type of inoculum and incubation time on
the cellulolytic activity of the extracts obtained was analyzed. Cellulases production after
15 days of incubation was higher than that obtained with 8 days of culture and it was
confirmed through an analysis of variance that the cellulases production for the mixed
and pure cultures of T. reesei on day 15 (34.5 and 31.9 U/g, respectively) did not present
significant differences. It was shown in this work that rice husks have the potential to be
used to obtain fungal enzymes, although the activity of enzymes obtained using this byproduct is lower than that produced with other cellulosic materials.
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